<p><strong style="-webkit-font-smoothing: antialiased; margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; font-variant-numeric: inherit; font-stretch: inherit; font-size: 14px; line-height: inherit; font-family: &quot;Helvetica Neue&quot;, Helvetica, Arial, sans-serif;">SIGNIFICANCE STATEMENT</strong><span style="font-family: &quot;Helvetica Neue&quot;, Helvetica, Arial, sans-serif; font-size: 14px;">&nbsp;We provide several important conceptual and technical advances in research on the PNS. We pioneer the first description of AMPA receptor (AMPAR)-mediated currents in the PNS glia of vertebrates and provide new insights into the properties of AMPAR channels in peripheral glia; for example, their Ca</span><span style="-webkit-font-smoothing: antialiased; font-size: 0.75em; position: relative; bottom: 0.5em; margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; font-variant-numeric: inherit; font-stretch: inherit; line-height: inherit; font-family: &quot;Helvetica Neue&quot;, Helvetica, Arial, sans-serif;">2+</span><span style="font-family: &quot;Helvetica Neue&quot;, Helvetica, Arial, sans-serif; font-size: 14px;">&nbsp;permeability and single-channel conductance. We describe for the first time the electrophysiological and morphological properties of Schwann cells (SCs) at different stages of development and show that functional AMPARs are expressed only in developing, not mature, SCs. Finally, we introduce a preparation of peripheral nerve slices for patch-clamp recordings. This preparation opens new possibilities for studying the physiology of SCs in animal models and in surgical human samples.</span><br></p>